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KMID : 0381120110330050535
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Genes and Genomics
2011 Volume.33 No. 5 p.535 ~ p.535
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Isolation and characterization of 12 microsatellite loci from Korean water deer (Hydropotes inermis argyropus) for population structure analysis in South Korea
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Lee Yun-Sun
Choi Sung-Kyoung
An Jung-Hwa
Park Han-Chan
Kim Sang-In
Min Mi-Sook
Kim Kyung-Seok
Lee Hang
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Abstract
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The main goals of this study were to isolate microsatellites markers of Korean water deer (Hydropotes inermis argyropus) and understand the genetic status of the species in South Korea. Twelve new microsatellite loci were isolated and characterized to establish basic population genetic parameters for 45 H. i. argyropus specimens in South Korea. There were no significant regional or genetic structure differences between the mid-eastern and southwestern populations in South Korea according to the population genetic analyses. The number of alleles per locus ranged from two to 13 with an average of 6.08. Mean expected (H E) and observed heterozygosity (H O) were 0.622 and 0.533, respectively. Microsatellite variability was also not significant between the two regions (F ST=0.012). These new markers should facilitate the future population genetics studies of Korean water deer and other closely related species.
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KEYWORD
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Korean water deer, Hydropotes inermis argyropus, Microsatellite, Population genetic, Genetic structure, Magnetic bead enrichment
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